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fbxo6  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology fbxo6
    Fbxo6, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fbxo6/FBXO6+Antibody/pmc10457387-183-76-78
    Average 93 stars, based on 10 article reviews
    fbxo6 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Overexpression of Fbxo6 inactivates spindle checkpoint by interacting with Mad2 and BubR1
    Article Snippet: Protein extracts were equally loaded on a 6–12% SDS-polyacrylamide gel, electrophoresed, and blotted on to Immobilon-NC membranes (Amersham Biosciences, Piscataway, NJ, USA). .. After blocking with 5% nonfat milk in PBS, membranes were incubated overnight at 4°C with mouse antibodies against Flag M2 (Sigma-Aldrich), cyclin B1 (Abcam, UK), Fbxo6, Cdc27, Cullin1 (Santa Cruz, CA), phospho-Histone H3 (Ser10) (Upstate Biotechnology Inc, Lake placid, NY) or with rabbit antibodies against Mad2 (Bethyl Laboratories Inc, Montgomery, TX), and BubR1 (Novus, Littleton, CO), Bub3 (Santa Cruz, CA), followed by incubation with horseradish peroxidase (HRP)-linked secondary antibodies (Cell Signaling, Beverly, MA). .. Specific signals were detected by chemiluminescence (Cell Signaling).

    Incubation:

    Article Title: Overexpression of Fbxo6 inactivates spindle checkpoint by interacting with Mad2 and BubR1
    Article Snippet: Protein extracts were equally loaded on a 6–12% SDS-polyacrylamide gel, electrophoresed, and blotted on to Immobilon-NC membranes (Amersham Biosciences, Piscataway, NJ, USA). .. After blocking with 5% nonfat milk in PBS, membranes were incubated overnight at 4°C with mouse antibodies against Flag M2 (Sigma-Aldrich), cyclin B1 (Abcam, UK), Fbxo6, Cdc27, Cullin1 (Santa Cruz, CA), phospho-Histone H3 (Ser10) (Upstate Biotechnology Inc, Lake placid, NY) or with rabbit antibodies against Mad2 (Bethyl Laboratories Inc, Montgomery, TX), and BubR1 (Novus, Littleton, CO), Bub3 (Santa Cruz, CA), followed by incubation with horseradish peroxidase (HRP)-linked secondary antibodies (Cell Signaling, Beverly, MA). .. Specific signals were detected by chemiluminescence (Cell Signaling).



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